期刊
SCIENCE
卷 335, 期 6076, 页码 1621-1623出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1214402
关键词
-
资金
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/D010799/1]
- EU [LSHG-CT-2006-037900]
- BBSRC [BB/G01406X/1, BBS/E/J/000CA447, BB/D010799/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G01406X/1, BB/D010799/1, BBS/E/J/000CA447] Funding Source: researchfish
Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据