期刊
RSC ADVANCES
卷 5, 期 21, 页码 16173-16189出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra07351g
关键词
-
资金
- MICINN [ENE201125188]
- SENECA Foundation [18975/JLI/13]
- Erasmus Mundus EU Mare Nostrum Program
The use of a wide range of water miscible and immiscible ionic liquids (ILs) as reaction media for ABTS (2,2'-azino- bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) oxidation by Trametes versicolor laccase was studied. Thirteen ILs were shown to be suitable media for the laccase oxidation reaction, increasing the activity with respect to conventional media. Among them, the water-miscible IL choline dihydrogen phosphate [Chol][H2PO4] allowed over-laccase activity with an enhancement rate of 451% at 25 degrees C and pH 7.0. This ionic liquid improved the stability of the enzyme in the face of high temperature and high pH, while storage at room temperature in aqueous medium was increased up to 4.5 times. Moreover, it was found that in the reaction medium for decolourizing dyes (antraquinonic and azoic) using laccase increased the decolourization rate by up to 216% and 137% for the azoic dyes Acid Black 1 and Remazol Brilliant Blue R, respectively. A high decolorization rate was also obtained for a mixture of dyes (80% within 8 h). To understand the effect of [Chol][H2PO4] on the secondary protein structure of the laccase, several spectroscopic techniques were used such as Circular Dichroism (CD), Fourier transform infrared (FT-IR) and Fluorescence, all of which demonstrated that the beta sheet structure was affected. A shift to an alpha-helix structure [Chol][H2PO4] could be responsible for the enhancement of the enzyme activity observed at 300 mM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据