期刊
SCIENCE
卷 332, 期 6037, 页码 1561-1564出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1205016
关键词
-
资金
- NIH [R01 GM38464, R01 GM93197]
- Center for Cancer Research, National Cancer Institute, NIH
- Division of Intramural Research of the NIH, National Institute of Environmental Health Sciences [Z01 ES065070, Z01 ES065089]
The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据