期刊
SCIENCE
卷 328, 期 5978, 页码 593-599出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1181348
关键词
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资金
- European Commission [LSHG-CT-2004-503464]
- Boehringer Ingelheim
- Vienna Spots of Excellence Programme
- Austrian Science Fund Special Research Programme Chromosome Dynamics
- Genome Research in Austria Programme
- Max Planck Society
- Bundesministerium fur Bildung und Forschung [NGFN-2 SMP-RNAi (01GR0402), NGFN-Plus (01GS0859)]
- Natural Science and Engineering Research Council of Canada [RGPIN-355644-2008]
- National Cancer Institute of Canada [019562]
- Human Frontier Science Program [CDA0044/200]
- Japan Society for the Promotion of Science
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of which had not or had only incompletely been characterized. This work has led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting complex and the g-tubulin ring complex-large complexes that are essential for spindle assembly and chromosome segregation. The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells.
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