期刊
SCIENCE
卷 328, 期 5983, 页码 1295-1297出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1188658
关键词
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资金
- National Institute of Allergy and Infectious Diseases, NIH [1R21NS067579-01, U54AI057141, KO8AI066251]
- NSF
- Swiss National Foundation [PA00P3-124140, PBBSA-120489, PA00P3-126243]
- Novartis Foundation
- Cystic Fibrosis Foundation [HOFFMA04L0]
- Swiss National Science Foundation (SNF) [PA00P3_126243, PA00P3_124140] Funding Source: Swiss National Science Foundation (SNF)
The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) regulates cellular motility and the synthesis of organelles and molecules that promote adhesion to a variety of biological and nonbiological surfaces. These properties likely require tight spatial and temporal regulation of c-di-GMP concentration. We have developed genetically encoded fluorescence resonance energy transfer (FRET)-based biosensors to monitor c-di-GMP concentrations within single bacterial cells by microscopy. Fluctuations of c-di-GMP were visualized in diverse Gram-negative bacterial species and observed to be cell cycle dependent. Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa. Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function.
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