期刊
SCIENCE
卷 327, 期 5966, 页码 685-689出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1182105
关键词
-
资金
- European Research
- European Molecular Biology Organisation
- Balliol College
- Oxford University
- Royal Society
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/H01991X/1, BB/E00458X/1] Funding Source: researchfish
- BBSRC [BB/E00458X/1, BB/H01991X/1] Funding Source: UKRI
The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread-the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据