期刊
SCIENCE
卷 326, 期 5959, 页码 1502-1509出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1176615
关键词
-
资金
- Canadian Cancer Society
- Canadian Institutes for Health [MOP-6849]
- Canada Foundation for Innovation
- Human Frontiers Science Program
- Lundbeck Foundation
- MRC [MC_U117532048] Funding Source: UKRI
- Medical Research Council [MC_U117532048] Funding Source: researchfish
Cells have self-organizing properties that control their behavior in complex tissues. Contact between cells expressing either B-type Eph receptors or their transmembrane ephrin ligands initiates bidirectional signals that regulate cell positioning. However, simultaneously investigating how information is processed in two interacting cell types remains a challenge. We implemented a proteomic strategy to systematically determine cell-specific signaling networks underlying EphB2-and ephrin-B1-controlled cell sorting. Quantitative mass spectrometric analysis of mixed populations of EphB2-and ephrin-B1-expressing cells that were labeled with different isotopes revealed cell-specific tyrosine phosphorylation events. Functional associations between these phosphotyrosine signaling networks and cell sorting were established with small interfering RNA screening. Data-driven network modeling revealed that signaling between mixed EphB2-and ephrin-B1-expressing cells is asymmetric and that the distinct cell types use different tyrosine kinases and targets to process signals induced by cell-cell contact. We provide systems- and cell-specific network models of contact-initiated signaling between two distinct cell types.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据