期刊
SCIENCE
卷 323, 期 5922, 页码 1743-1747出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1167525
关键词
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资金
- NIH [AI074420-02]
- Burroughs Wellcome Fund Investigator Award
- Hirschl Weill Caulier Career Scientist Award
- Mount Sinai School of Medicine Microscopy Shared Resource Facility [NIH-NCI 5R24 CA095823-04, NSF-DBI-9724504, NIH-S10RR09145-01]
- NSF Center for Biophotonics Science and Technology [PHY012099]
- UCD Health System Research Award
- UCD Clinical and Translational Science Center [NIH-NCRR ULRR024146]
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized buttons containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
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