期刊
RSC ADVANCES
卷 5, 期 125, 页码 103557-103562出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra24162f
关键词
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资金
- National Natural Science Foundation of China [21175155, 21475152]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20130162110017]
It is well known that Cu(II) can coordinate with L-alanine (Cu-Ala), which can be destroyed through the addition of glutamic-pyruvic transaminase (GPT) since GPT can effectively catalyze the conversion of L-alanine into keto-acetic acid. As a result, the free Cu(II) ion can combine with bovine serum albumin (BSA) and in turn quench the fluorescence of BSA. In this context, a simple and sensitive GPT activity detection via fluorescence quenching method has been developed. The fluorescence intensity of the system shows a linear relationship with the GPT concentration in the range of 5 and 400 U L-1 with a detection limit down to 3 U L-1 (S/N = 3). Avoiding any labels or complicated operations, this cost-effective and convenient method holds the potential for the rapid diagnosis of GPT-related diseases.
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