期刊
SCIENCE
卷 322, 期 5909, 页码 1845-1848出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1162228
关键词
-
资金
- NIH [GM25232]
RNA polymerases are highly regulated molecular machines. We present a method ( global run-on sequencing, GRO-seq) that maps the position, amount, and orientation of transcriptionally engaged RNA polymerases genome- wide. In this method, nuclear run- on RNA molecules are subjected to large- scale parallel sequencing and mapped to the genome. We show that peaks of promoter- proximal polymerase reside on similar to 30% of human genes, transcription extends beyond pre-messenger RNA 3 ' cleavage, and antisense transcription is prevalent. Additionally, most promoters have an engaged polymerase upstream and in an orientation opposite to the annotated gene. This divergent polymerase is associated with active genes but does not elongate effectively beyond the promoter. These results imply that the interplay between polymerases and regulators over broad promoter regions dictates the orientation and efficiency of productive transcription.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据