期刊
SCIENCE
卷 322, 期 5900, 页码 405-410出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1162609
关键词
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资金
- NIH [GM084279]
- Sandler Family Foundation
- Howard Hughes Medical Institute
- National Cancer Institute
- Center for Cancer Research
- California Institute of Quantitative Biology
- Ministry of Education, Science & Technology (MoST), Republic of Korea [KGM2420821] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
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