期刊
SCIENCE
卷 322, 期 5909, 页码 1855-1857出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1163853
关键词
-
资金
- Virginia and D. K. Ludwig Fund for Cancer Research
- NIH [CA57345, CA43460, CA62924, CA121113]
Transcription in mammalian cells can be assessed at a genome- wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts ( sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据