期刊
SCIENCE
卷 320, 期 5880, 页码 1185-1190出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1159151
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High complementarity between plant microRNAs ( miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA- guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA ( siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule- severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE ( VCS)/ Ge- 1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.
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