期刊
SCIENCE
卷 322, 期 5898, 页码 101-104出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1161847
关键词
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资金
- Agence Nationale de la Recherche [ANR-05-BLANC-0258-01]
- INRA
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence- based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17- billion- base- pair hexaploid bread wheat genome ( 2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome ( BAC)based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome- specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome- based approach.
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