期刊
SCIENCE
卷 321, 期 5896, 页码 1670-1673出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1160446
关键词
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资金
- Grant-in-Aid for Scientific Research (B)
- Grant-in-Aid for Scientific Research on Priority Areas Applied Genomics
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Urakami Foundation
- Skylark Food Science Institute
In microorganisms, menaquinone is an obligatory component of the electron- transfer pathway. It is derived from chorismate by seven enzymes in Escherichia coli. However, a bioinformatic analysis of whole genome sequences has suggested that some microorganisms, including pathogenic species such as Helicobacter pylori and Campylobacter jejuni, do not have orthologs of the men genes, even though they synthesize menaquinone. We deduced the outline of this alternative pathway in a nonpathogenic strain of Streptomyces by bioinformatic screening, gene knockouts, shotgun cloning with isolated mutants, and in vitro studies with recombinant enzymes. As humans and commensal intestinal bacteria, including lactobacilli, lack this pathway, it represents an attractive target for the development of chemotherapeutics.
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