期刊
SCHIZOPHRENIA RESEARCH
卷 124, 期 1-3, 页码 183-191出版社
ELSEVIER
DOI: 10.1016/j.schres.2010.07.002
关键词
MiRNA; Schizophrenia; Bipolar; Gene; Expression; TLDA
类别
资金
- SMRI [08R 1959]
- NIMH [2T32MH020030]
MicroRNAs (miRNAs) are a large family of small non-coding RNAs which negatively control gene expression at both the mRNA and protein levels The number of miRNAs identified is growing rapidly and approximately one-third is expressed in the brain where they have been shown to affect neuronal differentiation synaptosomal complex localization and synapse plasticity all functions thought to be disrupted in schizophrenia Here we investigated the expression of 667 miRNAs (miRBase v 13) in the prefrontal cortex of individuals with schizophrenia (SZ N=35) and bipolar disorder (BP N=35) using a real-time PCR-based Taqman Low Density Array (TLDA) After extensive QC steps 441 miRNAs were included in the final analyses At a FDR of 10% 22 miRNAs were identified as being differentially expressed between cases and controls 7 dysregulated in SZ and 15 in BP Using in silico target gene prediction programs the 22 miRNAs were found to target brain specific genes contained within networks overrepresented for neurodevelopment behavior and SZ and BP disease development In an initial attempt to corroborate some of these predictions we investigated the extent of correlation between the expressions of hsa-mir-34a -132 and -212 and their predicted gene targets mRNA expression of tyrosine hydroxylase (TH) phosphogluconate dehydrogenase (PGD) and metabotropic glutamate receptor 3 (GRM3) was measured in the SMRI sample Hsa-miR 132 and -212 were negatively correlated with TH (p = 0 0001 and 0 0017) and with PGD (p = 0 0054 and 0 017 respectively) Published by Elsevier B V
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