期刊
RSC ADVANCES
卷 5, 期 43, 页码 34342-34352出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra02489g
关键词
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资金
- National Natural Science Foundation of China [21202087, 81102374]
- National Basic Research Program of China (973 program) [2013CB911104]
- Fundamental Research Funds for the Central Universities [65124002]
- Tianjin Science and Technology Program [12JCQNJC08500, 13JCYBJC24300, 13JCQNJC13100]
- Specialized Research Fund for the Doctoral Program of Higher Education Ministry of Education of China [2120031120049]
- 111 Project of the Ministry of Education of China [B06005]
- State Key Laboratory of Elemento-Organic Chemistry of Nankai University
- Collaborative Innovation Center of Chemical Science and Engineering
The olanzapine-fluoxetine augmentation strategy has been proved efficacious for treatment-resistant depression, psychotic depression and bipolar depression. To achieve efficient therapeutic drug monitoring (TDM), we develop an automated online SPE-LC-MS/MS method for the simultaneous quantification of olanzapine, fluoxetine and norfluoxetine in human plasma. After adding an internal standard of diphenhydramine and centrifugation, a 10 mL plasma sample was directly injected into the SPE cartridge. While the analytes were retained on the SPE cartridge, the endogenous materials were washed out by the loading solvent. Following the valve switching, the analytes were eluted from the SPE cartridge to the analytical column by gradient elution. The analytes were quantified using a triple-quadrupole tandem mass spectrometer. Calibration curves were linear over the concentration range of 0.25-50.00 ng mL(-1) for olanzapine, and 0.50-100.00 ng mL(-1) for fluoxetine and norfluoxetine. The intra- and inter-day precisions were within 1.17% and 4.63%. And the accuracies were between 95.60% and 101.48%. The mean matrix effect was in the range of 90.35% to 99.89% and mean recovery was in the range of 90.35% to 96.99%. This method has been successfully applied to two Chinese schizophrenia patients. The online SPE-LC-MS/MS method allows sensitive and robust quantification of olanzapine, fluoxetine and norfluoxetine for routine TDM in clinic.
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