期刊
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
卷 78, 期 6, 页码 501-507出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00365513.2018.1512150
关键词
Inductively coupled plasma mass spectrometry; iodine; pregnant women
资金
- National Key Technology Research and Development Support program [2015BAI32H00]
- National Natural Science Foundation of China [81702060]
Rapid and simple method for serum and urine iodine is necessary for iodine-related diseases. This study aimed to establish and validate a simple and rapid method for quantification of iodine in human urine and serum using inductively coupled plasma mass spectrometry (ICP-MS). Generally, samples were diluted using water with 1.5% isopropanol and 7mmol hydrous ammonium before analysis by the ICP-MS. Performance of the method was validated, and the urine and serum iodine levels of 72 pregnant women were analyzed. Results showed that the lower limit of detection of the ICP-MS method was 0.87 mu g/L. No significant memory effects were observed. Recovery in both serum and urine was approximately 100%. Compared with NIST SRM3668, bias was <1%, exhibiting good accuracy. The inter-run coefficients of variation (CVs) for serum and urine iodine were 2.8-3.5% and 3.5-6.7%, respectively, and total CVs for serum and urine iodine were 3.2-3.7% and 3.9-6.7%, respectively. Comparison of the iodine results obtained by the ICP-MS method and the conventional S-K method revealed excellent correlation (r=0.984, mean bias=-1.7%). The median urine iodine of pregnant women (125.5g/L) was lower than that of WHO criteria for iodine deficiency (median urinary iodine <150g/L) while 55.6% of the individuals with urine iodine level less than 150g/L. The median serum iodine level of the pregnant women was 69.0g/L. In conclusion, a rapid and simple ICP-MS method for urine and serum iodine measurements has been established and validated, and the pregnant women in Beijing may still suffer from insufficient iodine intake.
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