期刊
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
卷 68, 期 7, 页码 548-554出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00365510701883180
关键词
Body fluid; ELISA; oral-based diagnostics; sample handling
资金
- Kornerup Fund
- Aase and Ejnar Danielsen Fund
- Danish Cancer Society
- IMK Foundation
- Danish Medical Research Counsel
- Familien Hede Nielsens Fund
- Danish Cancer Research Foundation
- Ib Henriksens Foundation
- Kathrine og Vigo Skovgaard Foundation
- Redaktor Kaaresens Foundation
- Obel Family Foundation
Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.
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