期刊
RSC ADVANCES
卷 5, 期 10, 页码 7285-7290出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra15065a
关键词
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资金
- National Natural Science Foundation of China [81160538, 81360658, 81260497]
- Research Fund for the Doctoral Program of Higher Education [20122201110001]
- Science and Technology Department of Jilin Province [20130206052YY]
Thymoquinone (TQ), a bioactive natural product obtained from the black cumin seeds of Nigella sativa Linn, is a widely used spice or herb. The present study investigated the hepatoprotective effect of TQ on acute hepatic failure induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice. The mice were intragastrically administrated TQ (5 or 20 mg kg(-1)) for 12 h and 1 h prior to D-GalN (700 mg kg(-1))/LPS (10 mg kg(-1)) injections and then sacrificed 8 h after treatment with D-GalN/LPS. TQ pretreatment reduced the mortality induced by D-GalN/LPS and reversed liver damage. TQ attenuated D-GalN/LPS-induced hepatocyte apoptosis, which was confirmed by suppressing caspase activation, PARP cleavage and the Bax/Bcl-2 ratio. Importantly, TQ attenuated the D-GalN/LPS-mediated phosphorylation of JNK, ERK and p38. Furthermore, TQ suppressed the production of proinflammatory cytokines. These findings suggested that TQ could modulate D-GalN/LPS-mediated acute hepatic failure by inhibiting caspase activation, consistent with the mitochondrial pathway of apoptosis and the MAPK signaling pathway.
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