期刊
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
卷 60, 期 3, 页码 431-436出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1021443713020179
关键词
Arabidopsis thaliana; IAA redistribution; GUS reporter; gas chromatography; mass-spectrometry; digital image analysis
资金
- Russian Foundation for Basic Research [08-04-00566, 11-04-00701]
- St. Petersburg Government [2.6/22-03/004, 2.6/16-05/219-A]
In the study of auxin transport, transgenic constructs, including DR5::GUS, are widely used for visualization of phytohormone localization. Previously we proposed a method for quantitative evaluation of the IAA content by histochemical staining for glucuronidase activity. In this work, this method was complemented by quantitative data on the content of IAA in plants obtained by gas chromatography-mass spectrometry (GC/MS), which allowed more accurate characterization of the lateral IAA gradient arising at the Arabidopsis thaliana (L.) Heynh (ecotype Columbia 0) root gravistimulation. Applied method of IAA analysis, combining GC/MS and histochemistry, can be used for quantitatification of the other plant hormone distribution in transgenic plants with the GUS reporter.
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