期刊
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
卷 60, 期 1, 页码 14-26出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S1021443712060131
关键词
marker-free transgenic plants; co-transformation; transposition; site-specific recombination; direct screening of transformants
资金
- Russian Foundation for Basic Research [10-04-00037, 11-08-00413, 12-08-00131]
The review considers the basic strategies used to produce biologically safe marker-free transgenic plants and analyzes their advantages and disadvantages. The systems of positive and negative selection as safer approaches for transformant identification are briefly described. The application of co-transformation, transposition, and site-specific recombination for production of marker-free plants is described. Special attention is paid to novel approaches to create marker-free plants initially containing no selective genes in their genomes.
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