期刊
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
卷 56, 期 5, 页码 709-715出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S1021443709050185
关键词
high-oil maize; SAD; 2-DE; gas chromatography; real-time PCR
资金
- National Nature Science Foundation of China [30571165]
- Agriculture Ministry of China [2004-Z25]
As special maize with more than 6% oil concentration in the grain, high-oil maize has received increased interest recently. To date, little is known about the expressions of genes involved in fatty acid metabolism of high-oil maize. Stearoyl-acyl carrier protein desaturase (SAD) is a key enzyme that converts stearic acid to oleic acid. In this study, two-dimensional electrophoresis, gas chromatography, and real-time PCR were used to determine the expressions of SAD at three seed development stages in high-oil and normal maize inbred lines. SAD was significantly more abundantly expressed in high-oil maize than in normal maize, not only at the protein and mRNA levels, but also at the product level. These results suggested that a high expression of SAD may play an important role in increasing oil concentration in high-oil maize.
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