期刊
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
卷 37, 期 2, 页码 244-248出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1068162011020099
关键词
oligo(2 '-O-methylribonucleotides); fluorescent probes; pyrene; pyrimidine nucleosides; mRNA of multidrug resistance gene mdr1
A series of novel fluorescent conjugates of oligo(2'-O-methylribonucleotides) containing insertions of 2'-bispyrenylmethylphosphorodiamidate derivatives of ribonucleosides (Urd, Cyd) were synthesized and their fluorescent properties were studied. The possibility to detect in solution the RNA transcript of the 5'-terminal fragment (1-678) of mRNA of the multidrug resistance gene mdr1 was demonstrated using synthesized multipyrene probes.
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