期刊
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
卷 34, 期 2, 页码 261-263出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1068162008020180
关键词
chromosome; dimeric bisbenzimidazole; DNA; fluorescent dye; HIV-1 integrase; inhibitor narrow groove ligand
Dimeric bisbenzimidazole DB(5) fluorescing in the blue spectral area (lambda(em) 476 nm) within the DNA complex was synthesized. DB(5) is bound by a double-strand DNA and can differentially stain human and plant (flax) chromosomes. According to preliminary data, it provides a considerably more intensive contrast of chromosome staining than DAPI and Hoechst 33258 dyes. It was also found that DB(5) is an in vitro inhibitor of HIV-1 integrase in the reaction of 3'-processing.
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