期刊
RNA
卷 16, 期 3, 页码 489-494出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.1701210
关键词
microRNA-140; microRNA target identification; Smad; TGF beta
资金
- European Commission [LSHG-CT-2006-037900]
- Fundacao Para a Ciencia e a Tecnologia [FCT] [SFRH/BD/33204/2007]
- Fundacao Calouste Gulbenkian, Siemens SA Portugal
- Fundação para a Ciência e a Tecnologia [SFRH/BD/33204/2007] Funding Source: FCT
mRNA profiling is routinely used to identify microRNA targets, however, this high-throughput technology is not suitable for identifying targets regulated only at protein level. Here, we have developed and validated a novel methodology based on computational analysis of promoter sequences combined with mRNA microarray experiments to reveal transcription factors that are direct microRNA targets at the protein level. Using this approach we identified Smad3, a key transcription factor in the TGF beta signaling pathway, as a direct miR-140 target. We showed that miR-140 suppressed the TGF beta pathway through repression of Smad3 and that TGF beta suppressed the accumulation of miR-140 forming a double negative feedback loop. Our findings establish a valid strategy for the discovery of microRNA targets regulated only at protein level, and we propose that additional targets could be identified by re-analysis of existing microarray datasets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据