期刊
RNA
卷 14, 期 3, 页码 454-459出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.603108
关键词
DsrA; mRNA stability; noncoding RNA; rpoS
资金
- Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)
- Biotechnology and Biological Sciences Research Council [BB/D016096/1] Funding Source: Medline
- BBSRC [BB/D016096/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D016096/1] Funding Source: researchfish
The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigma(S), includes translational activation by the noncoding RNA DsrA. We observed that the stability of rpoS mRNA, and concomitantly the concentration of sS, were significantly higher in an RNase III-deficient mutant. As no decay intermediates corresponding to the in vitro mapped RNase III cleavage site in the rpoS leader could be detected in vivo, the initial RNase III cleavage appears to be decisive for the observed rapid inactivation of rpoS mRNA. In contrast, we show that base-pairing of DsrA with the rpoS leader creates an alternative RNase III cleavage site within the rpoS/DsrA duplex. This study provides new insights into regulation by small regulatory RNAs in that the molecular function of DsrA not only facilitates ribosome loading on rpoS mRNA, but additionally involves an alternative processing of the target.
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