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Mechanistic insights from reversible splicing catalysis

期刊

RNA
卷 14, 期 10, 页码 1975-1978

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COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.1289808

关键词

ATPases; active site; conformational toggling; reversible splicing; spliced exon reopening

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Recent work demonstrating the ability of spliceosomes purified after the second catalytic step of splicing to efficiently reverse both steps of the reaction provides answers to several unresolved questions regarding the splicing reaction, and raises many more.

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