期刊
RNA BIOLOGY
卷 11, 期 8, 页码 1083-1095出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.35506
关键词
live-cell imaging systems; RNA nuclear foci; RNA localization; RNA fluorescence imaging; triplet repeat diseases
资金
- Ministry of Science and Higher Education [N N301 569340, N N302 633240]
- National Science Center [2011/03/B/NZ1/03259, 2012/06/A/NZ1/00094]
- European Regional Development Fund within Innovative Economy Program [POIG.01.03.01-30-098/08]
Numerous types of transcripts perform multiple functions in cells, and these functions are mainly facilitated by the interactions of the RNA with various proteins and other RNAs. Insight into the dynamics of RNA biosynthesis, processing and cellular activities is highly desirable because this knowledge will deepen our understanding of cell physiology and help explain the mechanisms of RNA-mediated pathologies. In this review, we discuss the live RNA imaging systems that have been developed to date. We highlight information on the design of these systems, briefly discuss their advantages and limitations and provide examples of their numerous applications in various organisms and cell types. We present a detailed examination of one application of RNA imaging systems: this application aims to explain the role of mutant transcripts in human disease pathogenesis caused by triplet repeat expansions. Thus, this review introduces live RNA imaging systems and provides a glimpse into their various applications.
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