期刊
RNA BIOLOGY
卷 11, 期 5, 页码 433-442出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.28301
关键词
small RNA GlmZ; glucosamine-6-phosphate synthase GlmS; RNase E adaptor protein RapZ (YhbJ); decoy small RNA GlmY; RNA mimicry; two-component system GlrK/GlrR (QseE/QseF)
资金
- DFG [SPP1258]
- German DFG [SPP1258]
In Escherichia coli, small RNAs GlmY and GlmZ feedback control synthesis of glucosamine-6-phosphate (GlcN6P) synthase GlmS, a key enzyme required for synthesis of the cell envelope. Both small RNAs are highly similar, but only GlmZ is able to activate the glmS mRNA by base-pairing. Abundance of GlmZ is controlled at the level of decay by RNase adaptor protein RapZ. RapZ binds and targets GlmZ to degradation by RNase E via protein-protein interaction. GlmY activates glmS indirectly by protecting GlmZ from degradation. Upon GlcN6P depletion, GlmY accumulates and sequesters RapZ in an RNA mimicry mechanism, thus acting as an anti-adaptor. As a result, this regulatory circuit adjusts synthesis of GlmS to the level of its enzymatic product, thereby mediating GlcN6P homeostasis. The interplay of RNase adaptor proteins and anti-adaptors provides an elegant means how globally acting RNases can be re-programmed to cleave a specific transcript in response to a cognate stimulus.
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