期刊
RNA BIOLOGY
卷 11, 期 7, 页码 836-844出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.29305
关键词
sorting signal; mRNA; extracellular vesicles
资金
- Spanish Ministry MICINN [PS09/00526, PI12-01604]
- ISCIII-Subdireccion General de Evaluacion
- European Fund for Regional Development (Feder)
- Program Ramon y Cajal of Spanish Ministry
- Basque Government [GV PI2012-45]
- Movember GAP1 Exosome Biomarker study
- Spanish ISCIII-MICINN
- National Science Centre, Poland [2012/05/B/ST6/03026]
Intercellular communication mediated by extracellular vesicles has proved to play an important role in normal and pathological scenarios. However not too much information about the sorting mechanisms involved in loading the vesicles is available. Recently, our group has characterized the mRNA content of vesicles released by hepatic cellular systems, showing that a set of transcripts was particularly enriched in the vesicles in comparison with their intracellular abundance. In the current work, based on in silico bioinformatics tools, we have mapped a novel sequence of 12 nucleotides C[TA]G[GC][AGT]G[CT]C[AT]GG[GA], which is significantly enriched in the set of mRNAs that accumulate in extracellular vesicles. By including a 3-UTR containing this sequence in a luciferase mRNA reporter, we have shown that in a hepatic cellular system this reporter mRNA was incorporated into extracellular vesicles. This study identifies a sorting signal in mRNAs that is involved in their enrichment in EVs, within a hepatic non-tumoral cellular model.
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