期刊
RNA BIOLOGY
卷 10, 期 5, 页码 817-827出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.24046
关键词
phage resistance; horizontal gene transfer; Cas; CRISPR; small RNA targets; crRNA; bioinformatics; R-loop
资金
- Royal Society
- VLAG (Voeding, Levensmiddelentechnologie, Agrobiotechnologie en Gezondheid)
- NWO-VIDI [864.11.005]
- Human Frontier Science Program Grant
- University of Otago
The bacterial and archaeal CRISPR/Cas adaptive immune system targets specific protospacer nucleotide sequences in invading organisms. This requires base pairing between processed CRISPR RNA and the target protospacer. For type I and II CRISPR/Cas systems, protospacer adjacent motifs (PAM) are essential for target recognition, and for type III, mismatches in the flanking sequences are important in the antiviral response. In this study, we examine the properties of each class of CRISPR. We use this information to provide a tool (CRISPRTarget) that predicts the most likely targets of CRISPR RNAs (). This can be used to discover targets in newly sequenced genomic or metagenomic data. To test its utility, we discover features and targets of well-characterized Streptococcus thermophilus and Sulfolobus solfataricus type II and III CRISPR/Cas systems. Finally, in Pectobacterium species, we identify new CRISPR targets and propose a model of temperate phage exposure and subsequent inhibition by the type I CRISPR/Cas systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据