期刊
ONCOTARGET
卷 6, 期 24, 页码 20555-20569出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.4111
关键词
miR-22; cutaneous T-cell lymphoma (CTCL); mycosis fungoides (MF); STAT3; STAT5; JAK3
资金
- Calsberg Foundation
- Novo Nordic Research Foundation
- Lundbeck Foundation
- Danish Research Council
- Danish Cancer Society (Kraeftens Bekaempelse)
- Dansk Kraeftforsknings Fond
- Novo Nordisk Fonden [NNF12OC0002036] Funding Source: researchfish
- The Danish Cancer Society [R72-A4571] Funding Source: researchfish
Aberrant activation of Janus kinase-3 (Jak3) and its key down-stream effectors, Signal Transducer and Activator of Transcription-3 (STAT3) and STAT5, is a key feature of malignant transformation in cutaneous T-cell lymphoma (CTCL). However, it remains only partially understood how Jak3/STAT activation promotes lymphomagenesis. Recently, non-coding microRNAs (miRNAs) have been implicated in the pathogenesis of this malignancy. Here, we show that (i) malignant T cells display a decreased expression of a tumor suppressor miRNA, miR-22, when compared to non-malignant T cells, (ii) STAT5 binds the promoter of the miR-22 host gene, and (iii) inhibition of Jak3, STAT3, and STAT5 triggers increased expression of pri-miR-22 and miR-22. Curcumin, a nutrient with anti-Jak3 activity and histone deacetylase inhibitors (HDACi) also trigger increased expression of pri-miR-22 and miR-22. Transfection of malignant T cells with recombinant miR-22 inhibits the expression of validated miR-22 targets including NCoA1, a transcriptional co-activator in others cancers, as well as HDAC6, MAX, MYCBP, PTEN, and CDK2, which have all been implicated in CTCL pathogenesis. In conclusion, we provide the first evidence that de-regulated Jak3/STAT3/STAT5 signalling in CTCL cells represses the expression of the gene encoding miR-22, a novel tumor suppressor miRNA.
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