期刊
ONCOTARGET
卷 6, 期 26, 页码 22812-22821出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.4333
关键词
acute myeloid leukemia; FLT3 internal tandem duplication; minimal residual disease; next-generation sequencing
资金
- Lille Cancer Research Institute
- Caisse d'Epargne de Flandres
- Siric OncoLille
- National Cancer Institute PRTK
Minimal Residual Disease (MRD) detection can be used for early intervention in relapse, risk stratification, and treatment guidance. FLT3 ITD is the most common mutation found in AML patients with normal karyotype. We evaluated the feasibility of NGS with high coverage (up to 2.4.10(6) PE fragments) for MRD monitoring on FLT3 ITD. We sequenced 37 adult patients at diagnosis and various times of their disease (64 samples) and compared the results with FLT3 ITD ratios measured by fragment analysis. We found that NGS could detect variable insertion sites and lengths in a single test for several patients. We also showed mutational shifts between diagnosis and relapse, with the outgrowth of a clone at relapse different from that dominant at diagnosis. Since NGS is scalable, we were able to adapt sensitivity by increasing the number of reads obtained for follow-up samples, compared to diagnosis samples. This technique could be applied to detect biological relapse before its clinical consequences and to better tailor treatments through the use of FLT3 inhibitors. Larger cohorts should be assessed in order to validate this approach.
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