期刊
ONCOTARGET
卷 7, 期 8, 页码 8726-8742出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.6474
关键词
microRNA; microRNA 125a; microRNA 125b; epithelial ovarian cancer; EIF4EBP1
资金
- Seoul National University Hospital Research Fund [03-2014-0280]
- Research Resettlement Fund for new faculty of Seoul National University
- Korea Gynecologic Cancer Bank through Bio & Medical Technology Development Program of the MSIP, Korea
The aim of the present study was to identify the specific miRNAs involved in regulation of EIF4EBP1 expression in ovarian cancer and to define their biological function. miRNA mimics and miRNA inhibitors were used in quantitative PCR, western blotting, and luciferase reporter assays to assess cell migration, invasiveness, and viability. miR-125a and miR-125b were downregulated in ovarian cancer tissue and cell lines relative to healthy controls. Increased expression of miR-125a and miR125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression. The inverse relationship between miR-125a and miR-125b was corroborated by cotransfection of a luciferase reporter plasmid. Furthermore, miR-125a and miR-125b caused apoptosis and decreased cell viability and migration in an apparently EIF4EBP1-directed manner. Collectively, these results indicate that miR-125a and miR-125b are important posttranscriptional regulators of EIF4EBP1 expression, providing rationale for new therapeutic approaches to suppress tumour invasion and migration using miR-125a, miR-125b, or their mimics for the treatment of ovarian cancer.
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