期刊
ONCOTARGET
卷 7, 期 5, 页码 5598-5612出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.6798
关键词
alpha-enolase; cancer metabolism; Warburg effect; cellular senescence
资金
- Associazione Italiana Ricerca sul Cancro [12182, 15257, 15232]
- Ministry of Health [RF-2013-02354892]
- University of Turin- Progetti Ateneo 2014-Compagnia di San Paolo (PC-METAIMMUNOTHER)
- Fondazione Ricerca Molinette
- Fondazione Ursula e Giorgio Cytron
- University of Turin- Progetti Ateneo 2014-Compagnia di San Paolo (PANTHER)
In the last 5 years, novel knowledge on tumor metabolism has been revealed with the identification of critical factors that fuel tumors. Alpha-enolase (ENO1) is commonly over-expressed in tumors and is a clinically relevant candidate molecular target for immunotherapy. Here, we silenced ENO1 in human cancer cell lines and evaluated its impact through proteomic, biochemical and functional approaches. ENO1 silencing increased reactive oxygen species that were mainly generated through the sorbitol and NADPH oxidase pathways, as well as autophagy and catabolic pathway adaptations, which together affect cancer cell growth and induce senescence. These findings represent the first comprehensive metabolic analysis following ENO1 silencing. Inhibition of ENO1, either alone, or in combination with other pathways which were perturbed by ENO1 silencing, opens novel avenues for future therapeutic approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据