期刊
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
卷 46, 期 1, 页码 97-99出版社
SOC BRASILEIRA MEDICINA TROPICAL
DOI: 10.1590/0037-868214142013
关键词
Diagnosis; WNV antibodies; ELISA
资金
- Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) [2006/01179-0]
Introduction: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. Methods: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. Results: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. Conclusions: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
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