期刊
NUTRIENTS
卷 7, 期 6, 页码 4383-4402出版社
MDPI AG
DOI: 10.3390/nu7064383
关键词
glioblastoma-initiating cells; RES; invasion; PI3K; Akt; NF-B; MMP-2
资金
- National Natural Science Foundation of China [81102463]
- China National Clinical Research Center for Neurological Diseases
- Capital Medical University Clinical-Basic Cooperation Research [11JL16]
- Training Plan for Beijing High-Level Healthcare Personnel [2011-3-28]
Invasion and metastasis of glioblastoma-initiating cells (GICs) are thought to be responsible for the progression and recurrence of glioblastoma multiforme (GBM). A safe drug that can be applied during the rest period of temozolomide (TMZ) maintenance cycles would greatly improve the prognosis of GBM patients by inhibiting GIC invasion. Resveratrol (RES) is a natural compound that exhibits anti-invasion properties in multiple tumor cell lines. The current study aimed to evaluate whether RES can inhibit GIC invasion in vitro and in vivo. GICs were identified using CD133 and Nestin immunofluorescence staining and tumorigenesis in non-obese diabetic severe combined immunodeficient (NOD/SCID) mice. Invasive behaviors, including the adhesion, invasion and migration of GICs, were determined by tumor invasive assays in vitro and in vivo. The activity of matrix metalloproteinases (MMPs) was measured by the gelatin zymography assay. Western blotting analysis and immunofluorescence staining were used to determine the expression of signaling effectors in GICs. We demonstrated that RES suppressed the adhesion, invasion and migration of GICs in vitro and in vivo. Moreover, we proved that RES inhibited the invasion of GICs via the inhibition of PI3K/Akt/NF-B signal transduction and the subsequent suppression of MMP-2 expression.
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