期刊
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
卷 20, 期 3, 页码 403-408出版社
SPRINGERNATURE
DOI: 10.1590/S0102-695X2010000300018
关键词
Eupafolin; hispidulin; HeLa cells; mitochondrial swelling
资金
- CNPq
- Fundacao Araucaria
- CAPES
- INCT de Processos Redox em Biomedicina-Redoxoma
The aim of this study was to determine whether eupafolin and hispidulin, flavones extracted from Eupatorium littorale Cabrera, Asteraceae, have the ability to change properties of biological membranes and promote cytotoxic effects. Eupafolin (50-200 mu M). decreased approximately 30% the rate and total amplitude of valinomycin induced swelling And 60-100% the energy-dependent mitochondrial swelling. Moreover, eupafolin (200 mu M) reduced 35% the mitochondrial permeability transition, and hispidulin did not change this parameter in any of the doses, tested. The evaluation of phase transition of DMPC liposomes with the probe DPH demonstrated that hispidulin and eupafolin affect gel and fluid phase. With mitochondrial membrane as model, hispidulin increased the polarization of fluorescence when used DPH-PA probe. Eupafolin and hispidulin (100 mu M) promoted a reduction of 40% in cellular viability of HeLa cells in 24 h. Our results suggest that eupafolin and hispidulin have cytotoxic effects that can be explained, in part, by alterations promoted On biological membranes properties and mitochondrial bioenergetics.
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