期刊
REVIEW OF SCIENTIFIC INSTRUMENTS
卷 84, 期 4, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4802678
关键词
-
资金
- NSERC PGS-D fellowship
- NSF [PHY-0748564]
- Direct For Mathematical & Physical Scien [0748564] Funding Source: National Science Foundation
The magnetic tweezer is a single-molecule instrument that can apply a constant force to a biomolecule over a range of extensions, and is therefore an ideal tool to study biomolecules and their interactions. However, the video-based tracking inherent to most magnetic single-molecule instruments has traditionally limited the instrumental resolution to a few nanometers, above the length scale of single DNA base-pairs. Here we have introduced superluminescent diode illumination and high-speed camera detection to the magnetic tweezer, with graphics processing unit-accelerated particle tracking for high-speed analysis of video files. We have demonstrated the ability of the high-speed magnetic tweezer to resolve particle position to within 1 angstrom at 100 Hz, and to measure the extension of a 1566 bp DNA with 1 nm precision at 100 Hz in the presence of thermal noise. (C) 2013 AIP Publishing LLC. [http://dx.doi.org/10.1063/1.4802678]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据