期刊
RETROVIROLOGY
卷 9, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1742-4690-9-87
关键词
SAMHD1; Quiescent CD4(+) T-cell; HIV-1; Reverse transcription; Restriction
类别
资金
- ERC [250333]
- Sidaction (fonds de dotation Pierre Berge)
- ANRS
- FRM equipe labellisee
- SIDACTION
- MESR
- Ecole de l'INSERM-Liliane Bettencourt
- FRM
- Areva
- Labex IBEID program
- Vaccine Research Institute
- European Research Council (ERC) [250333] Funding Source: European Research Council (ERC)
Background: Quiescent CD4(+) T lymphocytes are highly refractory to HIV-1 infection due to a block at reverse transcription. Results: Examination of SAMHD1 expression in peripheral blood lymphocytes shows that SAMHD1 is expressed in both CD4+ and CD8+ T cells at levels comparable to those found in myeloid cells. Treatment of CD4+ T cells with Virus-Like Particles (VLP) containing Vpx results in the loss of SAMHD1 expression that correlates with an increased permissiveness to HIV-1 infection and accumulation of reverse transcribed viral DNA without promoting transcription from the viral LTR. Importantly, CD4(+) T-cells from patients with Aicardi-Goutieres Syndrome harboring mutation in the SAMHD1 gene display an increased susceptibility to HIV-1 infection that is not further enhanced by VLP-Vpx-treatment. Conclusion: Here, we identified SAMHD1 as the restriction factor preventing efficient viral DNA synthesis in non-cycling resting CD4(+) T-cells. These results highlight the crucial role of SAMHD1 in mediating restriction of HIV-1 infection in quiescent CD4(+) T-cells and could impact our understanding of HIV-1 mediated CD4(+) T-cell depletion and establishment of the viral reservoir, two of the HIV/AIDS hallmarks.
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