期刊
RETROVIROLOGY
卷 8, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1742-4690-8-29
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-
类别
资金
- Medical Research Council, UK
- Guy's & St Thomas' Charity
- International Consortium for Novel Anti-Virals
Background: Elucidating mechanisms that promote HIV-1 transfer between CD4(+) T-lymphocytes and their subsequent loss is of importance to HIV-1 pathogenesis. We recently reported that whey acidic protein, ps20, promotes cell-free HIV-1 spread through ICAM-1 modulation. Since ICAM-1 is pivotal in cell conjugation and intercellular HIV-1 transfer, this study examines ps20 effects on HIV-1 spread between T lymphocytes. Results: We demonstrate intrinsic ps20 variability in primary CD4(+) T-lymphocyte clonal populations and a significant positive correlation between endogenous ps20 levels and virus transfer involving fusion resulting in a spreading infection that could be reversed by the addition of reverse transcriptase inhibitors. Blocking anti-ps20 antibody or siRNA mediated ps20 knockdown, significantly reduced virus transfer. Conversely, virus transfer was promoted by ectopic ps20 expression or by exogenous addition of recombinant ps20. A higher frequency of virological synapse formation was evident in cocultures of HIV-1 infected donor T-cells with ps20(high) v ps20(low/intermediate) targets. Blocking ps20 inhibited T-lymphocyte conjugate formation and ICAM-1 expression, and was as potent as ICAM-1 in inhibiting HIV-1 transfer. Conclusions: Therefore ps20 is a novel marker of CD4(+) T-cells rendered vulnerable to HIV-1 infection by regulating the fundamental biologic process of intercellular conjugate formation and consequently of potential importance in HIV-1 pathogenesis.
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