期刊
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
卷 177, 期 3, 页码 327-332出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resp.2011.05.011
关键词
Temperature; Afferents; Vagal nerve; Transient receptor potential cation; channels
资金
- Natural Science Foundation of China [30770797, 30700269]
Changes in airway temperature can result in respiratory responses such as cough, bronchoconstriction and mucosal secretion after cold exposure and hyperventilation after heat exposure. In the present investigation, we examined the activity of bronchopulmonary receptors in response to activators of thermo-sensitive transient receptor potential (TS-TRP) cation channels using an ex vivo rat lung preparation. Receptive fields in small bronchioles were probed with von Frey hair monofilaments, warm (50 degrees C) or cold (8 degrees C) saline or saline containing TS-TRP agonists. Among 233 fibers tested, 159(68.2%) responded to heat (50 degrees C). A large proportion of heat-responsive receptors (107/145) were also activated by capsaicin. Heat and capsaicin-evoked responses were both blocked by TRPV1 antagonist, capsazepine. Only 15.3% of airway receptors responded to cold, which was associated with sensitivity to TRPM8 agonist menthol but not to TRPA1 agonist cinnamaldehyde (CA). Moreover, cold-evoked responses was unaffected by TRPA1 antagonist HC-03001. Our observations suggest that TRPV1 and TRPM8 are involved in transducing heat and cold in the lower respiratory tract, respectively. (C) 2011 Elsevier B.V. All rights reserved.
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