期刊
RESEARCH ON CHEMICAL INTERMEDIATES
卷 35, 期 5, 页码 607-613出版社
SPRINGER
DOI: 10.1007/s11164-009-0061-5
关键词
C-phycocyanin; Photostability; Silica; Conformation
资金
- National Natural Science Foundation of China [20873005]
- Beijing Natural Science Foundation [2083028]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
- University of Science and Technology Beijing
Application of protein encapsulation silica materials requires biomolecules to maintain stabilization on potentially adverse industrial conditions. The C-phycocyanin (C-PC) is chosen as a probe to monitor influence of the protein encapsulation process and to study protein and matrix interaction and stability of protein in silica matrix. Both C-PCs in solution and in silicate matrix irradiated by ultraviolet rays can result in photodestruction, whereas the protein in the silica is less affected. The measured photodamage rate constant of C-PC in silica is 25 times slower than that of C-PC in buffer solution. However, lifetimes of C-phycocyanin in phosphate buffer and silica matrix are identical. These studies suggest that entrapment of C-PCs into silica matrixes not only can keep their biological activity and obviously also improve their photostability.
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