期刊
RESEARCH IN VETERINARY SCIENCE
卷 94, 期 3, 页码 602-609出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2012.10.026
关键词
Glutathione transferase; Fasciola hepatica; Vaccine; Liver
资金
- EU [FOOD-CT-2005-023025-DELIVER]
- BBSRC UK [BB/H009256/1]
- Junta de Andalusia [P07-AGR-02900]
- BBSRC [BB/H009256/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H009256/1] Funding Source: researchfish
Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n = 7) was unimmunized and uninfected; group 2 (n = 10) was immunized with adjuvant Quil A and infected; group 3 (n = 10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and gamma delta T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-gamma in the hepatic inflammatory infiltrates was very occasional. (C) 2012 Elsevier Ltd. All rights reserved.
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