期刊
RESEARCH IN VETERINARY SCIENCE
卷 93, 期 2, 页码 729-732出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2011.09.008
关键词
In vitro culture; mRNA; Goats; Ovarian follicles
资金
- National Council for Scientific and Technological Development (CNPq, Brazil) [474731/2007-3]
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Brazilian Innovation Agency (FINEP)
- CAPES
This study verified the in vitro effects of IGF-1, FSH or both on caprine preantral follicle development and mRNA levels encoding IGF-1, IGFR-1 and FSHR. Secondary follicles were cultured for six days with FSH, IGF-1 or IGF-1 + FSH. The results showed that IGF-1 and/or FSH addition did not influence follicular development for six days. The interaction between IGF-1 and FSH increased the mRNA levels of IGF-1 and FSHR, and FSH increased the expression of the IGFR-1 mRNA. Thus, IGF-1 and/or FSH increased IGF-1, IGFR-1 and FSHR mRNA levels in in vitro cultured caprine secondary follicles, but they did not influence their development after six days of in vitro culture. (c) 2011 Elsevier Ltd. All rights reserved.
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