期刊
RESEARCH IN VETERINARY SCIENCE
卷 93, 期 2, 页码 749-757出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2011.09.012
关键词
Mesenchymal stem cells; In vitro differentiation; Neuron-like cells; Cardiomyocyte-like cells; Minipig
资金
- Basic Science Research Program through the National Research Foundation (NRF)
- Ministry of Education, Science and Technology [2010-0010528]
- BioGreen 21, Republic of Korea [20070301034040]
- National Research Foundation of Korea [2010-0010528] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [20070301034040] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The present study investigated the potential of minipig bone marrow-mesenchymal stem cells (BM-MSCs) to differentiate in vitro into neuron- and cardiomyocyte-like cells. Isolated BM-MSCs exhibited a fibroblast-like morphology, expressed CD29, CD44 and CD90, and differentiated into osteocytes, adipocytes and chondrocytes. Upon induction in two different neuronal specific media, most of BM-MSCs acquired the distinctive morphological features and positively stained for nestin, neurofilament-M (NF-M), neuronal nuclei (NeuN), beta-tubulin, galactocerebroside (Gal-C) and glial fibrillary acidic protein (GFAP). Expression of nestin, GFAP and NF-M was further demonstrated by RT-PCR and RT-qPCR. Following cardiomyogenic induction, MSCs exhibited a stick-like morphology with extended cytoplasmic processes, and formed cluster-like structures. The expression of cardiac specific markers a-smooth muscle actin, cardiac troponin T, desmin and alpha-cardiac actin was positive for immunofluorescence staining, and further confirmed by RT-PCR and RT-qPCR. In conclusion, our results showed the in vitro differentiation ability of porcine BM-MSCs into neuron-like and cardiomyocyte-like cells. (c) 2011 Elsevier Ltd. All rights reserved.
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