期刊
RESEARCH IN VETERINARY SCIENCE
卷 90, 期 3, 页码 536-539出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2010.07.008
关键词
CyHV-3; Goldfish; Common carp; Carrier; Cohabitation; PCR
Cyprinid herpesvirus-3 (CyHV-3) has spread worldwide and has had a major impact on koi and common carp production. Previous studies on the host range of the CyHV-3 found that fish species other than koi and common carp are fully resistant to natural virus exposure. Recently, CyHV-3 was detected in goldfish (Carassius auratus auratus) that were in contact with CyHV-3 infected koi. In the present study, a specific RT-PCR product was amplified from the viral thymidine kinase gene in gills, intestine and brain tissues of CyHV-3 infected goldfish. This implied that CyHV-3 replicated in these goldfish. Also, in the presence of a stress factor such as temperature fluctuation, the CyHV-3 infected goldfish transmitted the virus to cohabitated naive SPF common carp. CyHV-3 DNA was detected in the cohabitated nave carp tissues by PCR. The results of this study demonstrate that goldfish is a carrier for CyHV-3, permit virus propagation, and disseminate the virus to susceptible carp causing the disease. (C) 2010 Elsevier Ltd. All rights reserved.
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