Pathogen associated molecular pattern-induced TNF, IL-1β, IL-6 and CXCL-8 production from feline whole blood culture

Whole blood culture (C-wb) is a method to evaluate leukocyte response to stimuli. We used C-wb to evaluate the inflammatory response to pathogen associated molecular patterns (PAMPs) in cats. Blood was collected from diluted with RPMI and stimulated with various concentrations of lipopolysaccharide (LPS), lipoteichoic acid (LTA), peptidoglycan (PG) or control (PBS). Multiple concentrations of LPS, LTA and PG significantly stimulated tumor necrosis factor (TNF), interleukin (IL)-1 beta and CXC chemokine ligand (CXCL)-8 in feline C-wb. All PAMPs failed to stimulate IL-6 production and PG failed to stimulate OCCL-8 production. Lipopolysaccharide was a more potent inducer of IL-1 beta and CXCL-8 than LTA or PG and LTA is a more potent inducer of CXCL-8 than PG. Based on these data, PAMPs from gram positive and negative bacteria induce TNF, IL-1 beta and CXCL-8 production in feline whole blood. Cats appear to be relatively more sensitive to gram negative compared to gram positive bacteria. (c) 2010 Elsevier Ltd. All rights reserved.