Pharmacokinetics of florfenicol after intravenous and intramuscular administration in New Zealand White rabbits

The pharmacokinetic disposition and bioavailability of florfenicol (FF) were determined after single intravenous (i.v.) and intramuscular (i.m.) administrations of 25 mg/kg b.w. to ten healthy New Zealand White rabbits. Plasma FF concentrations were determined by high-performance liquid chromatography (HPLC). The plasma pharmacokinetic values for FF were best described by a one-compartment open model. The elimination half-life (t(1/2 beta)) was different (p < 0.05) however, the area under curve (AUC) was similar (p > 0.05) after i.v. and i.m. administrations. FF was rapidly eliminated (t(1/2 beta) 1.49 +/- 0.23 h), slowly absorbed and high (F, 88.75 +/- 0.22%) after i.m. injection. In addition, FF was widely distributed to the body tissues (V-ss 0.98 +/- 0.05 L/kg) after im. injection. In this study the time that plasma concentration exceeded the concentration of 2 mu g/mL was approximately 6 h. For bacteria with MIC of 2 mu g/mL, frequent administration at this dose would be needed to maintain the concentration above the MIC. However, it is possible that rabbit pathogens may have MIC values less than 2 mu g/mL which would allow for less frequent administration. Further studies are necessary to identify the range of MIC values for rabbit pathogens and to identify the most appropriate PK-PD parameter needed to predict an effective dose. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.